Anti-CD47 [h4C1]

Absolute Antibody

Anti-CD47 [h4C1]

7

This antibody binds the FG loop of the human CD47, which interacts with SIRPalpha and several other known CD47/SIRPalpha-blocking mAb. CD47 is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epith

This antibody specifically binds to the CD47 molecule to block the binding of CD47 to SIRPalpha, thereby activating macrophages and killing tumor cells. The blocking activity was demonstrated using flow cytometry assay. This antibody also bound CD47 ectodomain in an ELISA. Surface plasmon resonance (SPR)-binding assays demonstrated that h4C1 bound to the human CD47-ECD antigen with a KD of 0.85 nM. Macrophage-mediated phagocytosis activity was investigated by a tumor cell phagocytosis assay with bone marrow-derived macrophages (BMDMs) and tt was reported that h4C1 exhibited significant phagocytosis in Raji cells. In in vivo experiments this antibody improved survival and enhanced suppression of Raji cell xenografts in mice (PMID: 32296041). This antibody was also used in the generation of a bispecific antibody (BsAb) in a dual-variable-domain immunoglobulin (DVD-Ig) format targeting CD47 and PD-L1. The variable-domain from the PD-L1-blocking mAb #18 was introduced in tandem onto h4C1 to generate a dual variable domain bsAb that binds bivalently to both CD47 and PD-L1. This BsAb retained retained blocking activity for both interactions but lacked the hemagglutinating activity of the parental m4C1 (PMID: 32296041; 33244513).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELISA; FC; Block

Research Use Only

Monoclonal

h4C1

CD47

CD47 molecule

antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Rh-related antigen

Mouse

IgG2a

Leukocyte surface antigen CD47

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original mouse antibody was generated by immunizing BALB/c mice with human CD47-ecto recombinant protein emulsified with complete Freund's adjuvant. Later on the original mouse antibody was used to generate the humanized version of the antibody using sequence homology and computational prediction.

-20°C,2°C to 8°C

12352203