Anti-CD47 [mIAP301]

Absolute Antibody

Anti-CD47 [mIAP301]

7

MIAP301 binds specifically to the extracellular domain of CD47 (exon 2 that encodes both the predicted signal peptide clevage site and the entire Ig domain). CD47 plays a role in cell adhesion by acting as an adhesion receptor for THBS1 on platelets and i

MIAP301 can block murine CD47 interactions with SIRPalpha and functionally blocks CD47 in vivo in murine soft tissue ischemia and skin graft models (Soto-Pantoja et al. 2012). This antibody has also been used for FC (Olsson et al. Blood. 2005 May 1; 105(9): 3577-3582.). MIAP301 has also been shown to block KCl-evoked glutamate release from the hippocampus in mice, inhibiting glutamate release and thus impair memory retention (Chang et al. 2001).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, Neutralisation/Blocking

WB; IP; IF; FC; block

Research Use Only

Monoclonal

mIAP301

Cd47

CD47 antigen (Rh-related antigen, integrin-associated signal transducer)

9130415E20Rik; AA407862; AI848868; AW108519; B430305P08Rik; IAP; integrin-associated protein; It; Itgp; leukocyte surface antigen CD47; Rh-related antigen

Mouse

IgG2b

Leukocyte surface antigen CD47

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

MIAP301 was prepared by immunizing rats with intact CD47 purified from mouse placenta.

-20°C,2°C to 8°C

12352203