Anti-CD49E [SAM1]

Absolute Antibody

Anti-CD49E [SAM1]

9

This antibody is specific for human CD49E. CD49E is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. CD49E binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) an

To determine the potential of human antibodies for use in an endocap-based approach, immunohistochemistry was used on snap-frozen samples of normal liver, hepatocellular cancer, liver metastases of pancreatic cancer, normal pancreas and primary pancreatic cancer using the mouse version of this antibody. Furthermore, the cytotoxic properties of this antibody were determined in a resazurin viability assay on HUVECs and two different tumour cell lines (Wang et al, 2019; pmid:31398471). To evaluate which ligands on the neutrophil mediate adhesion to VCAM-1, flow cytometry was performed on human neutrophils using the mouse version of this antibody (Lomakina and Waugh, 2009; pmid:19134480). To determine the interaction of cells with titanium, immunofluorescence was used on human osteoblastic cells with the mouse version of this antibody. Furthermore, this antibody was used for flow cytometry on MG-63 cells (Luethen et al, 2005; pmid:15585246). To study how megakaryocytes differentiate and release platelets in the blood, immunofluorescence was performed on megakaryocytes using the mouse version of this antibody (Malara et al, 2011; pmid:21131589). To determine the regulatory effect of interleukin-4 (IL-4) on the expression and function of lymphocyte adhesion receptors involved in IL-2-induced cell aggregation, flow cytometry on peripheral blood lymphocytes using the mouse version of this antibody was performed. Furthermore, peripheral blood lymphocytes were used for immunofluorescence using this antibody (Vyth-Dreese et al, 1993; pmid:8473014).

Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry

Research Use Only

Monoclonal

SAM1

ITGA5

integrin subunit alpha 5

CD49 antigen-like family member E; CD49e; fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; integrin alpha-5; integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; very late activation protein 5, alpha subunit; VLA-5; VLA5A

Human

IgG1

Integrin alpha-5

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203