Anti-CD50 [101-1D2], Rabbit IgG, kappa

Absolute Antibody

Anti-CD50 [101-1D2], Rabbit IgG, kappa

9

This antibody recognizes CD50 domain 1.

The original version of this antibody was used for immunofluorescence on T cells, B cells and non lymphoic cell lines (Juan et al., 1993; PMID:8325327), on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), and on peripheral blood stimulated or unstimulated cells . Furthermore this antibody was used for flow cytometry on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), on peripheral blood stimulated or unstimulated cells (Vilella et al., 1990; PMID:1710077), and on T cells (Vilardell et al., 1998; PMID: 9672149). This antibody was also used for immunoprecipitation on PMBCs to determine whether CD50 and ICAM-3 are the same protein (Juan et al., 1993; PMID:8325327), and on peripheral blood stimulated or unstimulated cells to prove that 101-1D2 and 140-11 recognize the same molecule (Vilella et al., 1990; PMID:1710077). This antibody was used for immunohistochemistry on human neoplasms to study the expression of CD50 (Terol et al., 1996; PMID: 8946680). The ability of this antibody to inhibit cell-mediated lympholysis was tested in an inhibition assay on PMBCs was tested, it showed it was able to inhibit lympholysis (Vilella et al., 1990; PMID:1710077). Finally this antibody was used for western blot on immunoprecipitated proteins. This experiment shows that the CD50 protein coprecipitates with a 56-kD lck protein and a 59-kD fyn PTK (Juan et al., 1994; PMID:7515097).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

101-1D2

ICAM3

intercellular adhesion molecule 3

CD50; CDW50; ICAM-3; ICAM-R; intercellular adhesion molecule 3

Rabbit

IgG

Intercellular adhesion molecule 3

Human

-20°C,2°C to 8°C

12352203