Anti-CD53 [OX-44]

Absolute Antibody

Anti-CD53 [OX-44]

7

This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is

This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12% of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-

Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

FC; IHC-Fr; IHC-P; IP

Research Use Only

Monoclonal

OX-44

Cd53

Cd53 molecule

CD53 antigen; cell surface glycoprotein CD53; leukocyte antigen MRC OX-44; leukocyte surface antigen CD53; OX44; Ox-44

Rabbit

IgG

Leukocyte surface antigen CD53

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Rat

Rat

This antibody was prepared by immunizing BALB/c mice with T lymphoblasts from an MLR between purified rat T helper cells and irradiated semiallogeneic rat spleen.

-20°C,2°C to 8°C

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