Anti-CD56 ANK-1 [8G6], Rat IgM, kappa

Absolute Antibody

Anti-CD56 ANK-1 [8G6], Rat IgM, kappa

9

This antibody binds an epitope called ANK-1 present on most NCAM isoforms expressed on a subset of NK cells of mouse and human. This epitope is selectively expressed on pre-activated, adherent natural killer NK cells (pre-A-NK cells), which are interleuki

This antibody was used to classify the nonactivated human peripheral blood NK cells expressing ANK-1 epitope using flow cytometry. It was reported that ANK-1 epitope was preferentially expressed by around 26% of NK cells (range 9-56%) and was exclusively CD56dim. This antibody was also capable of detecting ANK-1 epitope from crude human peripheral blood NK cell and affinity purified brain N-CAM lysates using western blot. This antibody was also used to test the ability of NK cells to respond to IL-2 by generation and expansion of A-NK cells with LAK activity, following depletion of ANK-1+ NK cells by treatment with mAb ANK-1 and complement. This antibody can also be used for the immunohistochemical staining of paraformaldehyde fixed paraffin embedded tissues of spleen, lymph nodes, tonsils, intestines, lungs, and liver tissues (PMID: 15356097).

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

8G6

Ncam1

neural cell adhesion molecule 1

CD56; E-NC; E-NCAM; NC; Ncam; N-CAM-1; NCAM-1; neural cell adhesion molecule 1

Rat

IgM

Neural cell adhesion molecule 1

Human, Mouse

-20°C,2°C to 8°C

12352203