Anti-CD62L [OX-85]

Absolute Antibody

Anti-CD62L [OX-85]

7

OX-12 binds to rat antibody kappa light chains - has preference for IgKa allotype.

This antibody binds to CD62L expressed on rat lymphocytes, and has been used to show that recombinant soluble rat CD62L-CD4, to which it also binds, is likely to be correctly folded (Nicholson, 1998). This antibody also labels, and can therefore be used to fractionate, the subpopulation of rat CD4+CD8- thymocytes that expresess L-selectin (Seddon, 1996). Cells with this phenotype are able to protect rats from diabetes upon adoptive transfer, as well as providing B cells with help for secondary antibody responses and mediating local graft-versus-host reactions.

Flow Cytometry, ELISA

FC; ELISA

Research Use Only

Monoclonal

OX-85

Rabbit

IgG

L-selectin

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Rat

Rat

This antibody was prepared by immunizing 8 –12-week-old BALB/c mice with purified rat CD62L-CD4

-20°C,2°C to 8°C

12352203