Anti-CD8 alpha/Lyt-2 [YTS 169.4]

Absolute Antibody

Anti-CD8 alpha/Lyt-2 [YTS 169.4]

9

This antibody recognises the murine CD8 cell surface antigen expressed by a subset of T lymphocytes.

The antibody has been shown to show depleting activity in vivo. Mice have two alleles for CD8, Lyt2.1, and Lyt2.2, which are restricted to certain mouse strains. Lyt2.1, for example, is expressed in the mouse strains CBA, AKR, C3H, and DBA, whereas Lyt2.2 is expressed in the mouse strains BALB/c and C57BL/6 (B/6). YTS 169.4 was originally raised against CBA mouse thymocytes. However, there are publications in which it has been used in C57BL/6 and BALB/c mice, wherein it was used specifically as a pan-reactive Lyt2 reagent (Tavare et al., 2014; PMID: 24390540).

Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application

Research Use Only

Monoclonal

YTS 169.4

Cd8a

CD8 antigen, alpha chain

BB154331; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2

Mouse

IgG2a

T-cell surface glycoprotein CD8 alpha chain

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Mouse

12352203