Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody(M02236-1) CD8A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8A Antibody (M02236-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody(M02236-1) CD8A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8A Antibody (M02236-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-CD8 CD8A Rabbit Monoclonal Antibody
M02236-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetCD8A
Overview
- SupplierBoster Bio
- Product NameAnti-CD8 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEAF-3
- FormulationLiquid
- Gene ID925
- Target nameCD8A
- Target descriptionCD8a molecule
- Target synonymsCD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
- HostRabbit
- IsotypeIgG
- Protein IDP01732
- Protein NameT-cell surface glycoprotein CD8 alpha chain
- Scientific DescriptionBoster Bio Anti-CD8 CD8A Rabbit Monoclonal Antibody catalog # M02236-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
- ReactivityHuman, Rat
- Storage Instruction-20°C
- UNSPSC12352203