Anti-CD80 [#112]

Absolute Antibody

Anti-CD80 [#112]

9

This antibody is specific for chicken CD80, a type I glycoprotein consisting of two extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail. Functionally, CD80 on antigen presenting cells (APCs) binds to CD28 on T cells,

This antibody was used for detection of chicken CD80 expressed on CHO cells by flow cytometry; it was also used to detect CD80 on monocytes in the bursa of Fabricius and on spleen monocytes. chCD80 from transfected CHO cells, or from LPS-stimulated mononuclear lymphocytes were used for immunoprecipitation. In this procedure the protein was immunoprecipitated using this antibody. This antibody was used for immunohistochemistry on the bursa of Fabricius and spleen. Immunofluorescence was performed on chicken intestinal dendritic cells using this antibody. The antibody inhibited ConA-stimulated spleen cell differentiation into MHCII+ cells and blocked IL-2 induced spleen blast cell proliferation (Lee et al, 2011; pmid: 21334748).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry

Research Use Only

Monoclonal

#112

CD88

CD88 molecule

CD80; CD80 antigen; CD80 molecule; T-lymphocyte activation antigen CD80

Mouse

IgG1

CD80 protein

This chimeric mouse antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

Chicken

-20°C,2°C to 8°C

12352203