Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody (M00220-1) CD86 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD86 Antibody (M00220-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody (M00220-1) CD86 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD86 Antibody (M00220-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-CD86/B7 2 Rabbit Monoclonal Antibody
M00220-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCD86
Overview
- SupplierBoster Bio
- Product NameAnti-CD86/B7 2 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBFF-3
- FormulationLiquid
- Gene ID942
- Target nameCD86
- Target descriptionCD86 molecule
- Target synonymsB7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1; LAB72; T-lymphocyte activation antigen CD86
- HostRabbit
- IsotypeIgG
- Protein IDP42081
- Protein NameT-lymphocyte activation antigen CD86
- Scientific DescriptionBoster Bio Anti-CD86/B7 2 Rabbit Monoclonal Antibody catalog # M00220-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Sanguinarine, identified as a natural alkaloid LSD1 inhibitor, suppresses lung cancer cell growth and migration. Qin TT et al., 2022 Jun, Iran J Basic Med SciRead more
- Whartons jelly mesenchymal stem cell-derived small extracellular vesicles as natural nanoparticles to attenuate cartilage injury via microRNA regulation. Chen P et al., 2022 Jul 25, Int J PharmRead more
- FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis. Wang Y et al., 2022 May 23, JCI InsightRead more
- Growth differentiation factor-15 promotes immune escape of ovarian cancer via targeting CD44 in dendritic cells. Gao Y et al., 2021 May 1, Exp Cell ResRead more
- CPEB3 inhibits epithelial-mesenchymal transition by disrupting the crosstalk between colorectal cancer cells and tumor-associated macrophages via IL-6R/STAT3 signaling. Zhong Q et al., 2020 Jul 11, J Exp Clin Cancer ResRead more