Anti-CD89 [14A8 (14.1)]

Absolute Antibody

Anti-CD89 [14A8 (14.1)]

9

This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site.

This antibody binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.The binding of this antibody to human CD89 was confirmed with ELISA. This antibody was also capable of recognizing human CD89 expressing cells like human PMNL cells by flow cytometry. This antibody is not capable of blocking binding of IgA to CD89. This antibody can used to immunoprecipitate soluble CD89 (US7192582). A bispecific antibody construct incorporating the variable regions of the anti-gp41 antibody F240 and the anti-CD89 (immunoglobulin A [IgA] receptor) antibody 14A8 was reported to promote destruction of HIV type 1 (HIV-1) by neutrophils (PMID: 18272577). A Fab-like bispecific antibody molecule or Bi-Fab antibody generated from F240scFv and 14A8scFv showed significant antiboy dependent cell-mediated viral inhibition (ADCVI) activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection (PMID: 27419146).

Flow Cytometry, ImmunoPrecipitation, ELISA

Research Use Only

Monoclonal

14A8 (14.1)

FCAR

Fc fragment of IgA receptor

CD89; CTB-61M7.2; Fc alpha receptor; Fc fragment of IgA, receptor for; FcalphaRI; FCAR variant 14; immunoglobulin alpha Fc receptor

Human

IgG1

Immunoglobulin alpha Fc receptor

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203