Anti-CD8a [VHHCD8], Rabbit IgG-Fc Fusion,

Absolute Antibody

Anti-CD8a [VHHCD8], Rabbit IgG-Fc Fusion,

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The antibody is specific for CD8.

The binding affinity of the original format of the antibody to CD8 was measured by surface plasmon resonance (Kd= 0.9 nM). FACS analysis of splenocytes and lymph nodes gated on CD45+CD19-CD3+ cells, confirming that the original format of the antibody stained CD8+ cells. The antibody competed with a commercial anti-CD8alpha antibody, showing it bound to the CD8alpha chain. 89Zr-PEGylated antibody was used for PET imaging on mice, to track the presence of intratumoral CD8+ T cells (Rashidian et al., 2017; PMID: 28666979).

Flow Cytometry, Other Application

Research Use Only

Monoclonal

VHHCD8

Cd8a

CD8 antigen, alpha chain

BB154331; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2

Rabbit

IgG

T-cell surface glycoprotein CD8 alpha chain

This is a chimeric antibody, developed from the original camelid VHH antibody, specifically engineered for enhanced compatibility with existing reagents, assays, and techniques.

Mouse

-20°C,2°C to 8°C

12352203