Anti-CD8A [YCATE55.9]

Absolute Antibody

Anti-CD8A [YCATE55.9]

9

This antibody is specific for the alpha chain of canine CD8, and reacts with a rat cell line transfected with cDNA for canine CD8alpha (Gorman, 1994)

This antibody recognizes canine CD8alpha, and has been used in in IF and IHC studies to establish that CD8 expression is confined to a minority population of T cells, as is the case for other species (Cobbold, 1994). In a canine transplant model, administration of this antibody, in combination with another anti-CD8 antibody (YCATE60.3.9) causes a 90 per cent depletion of CD8+ T cells from the peripheral blood, but does not result in prolonged allograft survival (Watson, 1993). However, the administration of this antibody in combination with an anti-CD4 antibody is associated with an immunosuppressive effect, and an extended median graft survival time. This antibody blocks MHC class I dependant T-cell responses in vitro and in vivo.

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

YCATE55.9

CD8A

CD8a molecule

CD8 antigen, alpha polypeptide (p32); T-cell surface glycoprotein CD8 alpha chain

Canine

IgG2

T-cell surface glycoprotein CD8 alpha chain

This chimeric dog antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Canine

-20°C,2°C to 8°C

12352203