Anti-CD98 [8-34B]

Absolute Antibody

Anti-CD98 [8-34B]

9

The antibody is specific for CD98.

The murine version of the antibody inhibited tumor growth in Ramos xenografts and AML (OCI-AML-3) xenografts. Further, the chimeric version of the antibody was tested, confirming that also this version of the antibody could inhibit in vivo tumor growth with efficacy comparable to their parental counterparts. The antibody showed positive staining in 10/15 malignant melanomas and 4/18 lung cancers. In addition, the antibody gave positive staining in 6 of 6 frozen lung cancer samples and 2 of 2 frozen melanoma samples. Binding affinities of the murine and chimeric version of the antibody were determined using FACS analysis (Kd values between 0.9 nM and 4.5 nM). The chimeric antibody was also subjected to FACS analysis using three AML primary tumor samples and one cell line expressing cynomolgus monkey CD98 (cynCD98). The results demonstrated that the chimeric retained binding to human CD98 on AML cells and on cynCD98. The binding affinity of the murine, chimeric and humanized version of the antibody to CD98 was measured by surface plasmon resonance (JP2015501639A5).

Flow Cytometry, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

8-34B

SLC7A5

solute carrier family 7 member 5

4F2 light chain; 4F2LC; CD98; CD98 light chain; D16S469E; E16; integral membrane protein E16; large neutral amino acids transporter small subunit 1; LAT1; L-type amino acid transporter 1; MPE16; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (amino acid transporter light chain, L system), member 5; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5

Mouse

IgG1

Large neutral amino acids transporter small subunit 1

Human

-20°C,2°C to 8°C

12352203