Anti-CD99 [12E7]

Absolute Antibody

Anti-CD99 [12E7]

9

This antibody binds human CD99. CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. It plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membran

The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewings sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). This antibody was also used in a study relating to the cell surface expression MIC2 antigen on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting (PMID: 7506950).

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, RadioImmunoAssay

Research Use Only

Monoclonal

12E7

CD99

CD99 molecule (Xg blood group)

antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; surface antigen MIC2; T-cell surface glycoprotein E2

Rabbit

IgG

CD99 antigen

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203