Anti-Cholera enterotoxin B subunit [TE33]

Absolute Antibody

Anti-Cholera enterotoxin B subunit [TE33]

9

This antibody binds the CTP3 loop of the B subunit of the cholera toxin. This antibody is not capable of binding cholera toxin in its native form or its wild type form. TE33 specifically recognizes a loop comprising the sequence VPGSQHID and a beta-turn f

The original IgG1 antibody which was raised against the CTP3 peptide also cross reacts with cholera toxin in a solid phase ELISA. The binding of the original IgG1 antibody to cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli was done using solid-phase RIA and western blot (PMID: 2450576). In solution, the original antibody has a thousandfold lower affinity for the cholera toxin as compared to that for CTP3 (PMID: 1379072). The binding of the original antibody to both CTP3 peptide and cholera toxin was assayed by ELISA, solid-phase radioimmunoassay and immunoblot assay. The antibody was incapable of binding cholera toxin in its native form when tested in a solution-phase assay system (PMID: 1993848).

Western Blot, ELISA, RadioImmunoAssay

Research Use Only

Monoclonal

TE33

ctxB

cholera enterotoxin binding subunit CtxB

cholera enterotoxin binding subunit CtxB; VC_1456; VC_RS07050; VC1456

Rabbit

IgG

Cholera enterotoxin subunit B

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203