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Figure 1. Western blot analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody (PB9114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CIAS1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PB9114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIAS1/NALP3 at approximately 37 kDa. The expected band size for CIAS1/NALP3 is at 37 kDa.
Figure 1. Western blot analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody (PB9114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CIAS1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PB9114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIAS1/NALP3 at approximately 37 kDa. The expected band size for CIAS1/NALP3 is at 37 kDa.
Figure 1. Western blot analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody (PB9114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CIAS1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PB9114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIAS1/NALP3 at approximately 37 kDa. The expected band size for CIAS1/NALP3 is at 37 kDa.

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband(r)

Research Use Only
PB9114-HRP
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNLRP3
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CIAS1/NALP3/NLRP3 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    WB: The detection limit for CIAS1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    HRP
  • Gene ID114548
  • Target name
    NLRP3
  • Target description
    NLR family pyrin domain containing 3
  • Target synonyms
    AGTAVPRL; AII; AVP; C1orf7; caterpiller protein 1.1; CIAS1; CLR1.1; cold autoinflammatory syndrome 1 protein; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96P20
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 3
  • Scientific Description
    Boster Bio Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® catalog # PB9114. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203