Anti-CMV glycoprotein gB [HCMV37]

Absolute Antibody

Anti-CMV glycoprotein gB [HCMV37]

7

This antibody immunoprecipitates gB from HCMV infected cells and neutralizes HCMV infectivity in vitro. Immunofluorescence was used to study whether HCMV gB synthesized in infected cells is transported to the cell surface (PMID: 3024973). Humanized version of this antibody was made by grafting technique. Significant restoration of binding was obtained by substitution of human VH amino acids Thr28, Phe29, Ser30 with murine residues Ser28, Ile29, Thr30, in conjunction with the position 94 change (PMID: 7772562). This antibody was used in detection of gB in an ELISA (PMID: 22836657).

ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking

NTRL; ELISA; IHC-Fr; IF; ICC

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203