Figure 1. IHC analysis of CNTF using anti-CNTF antibody (PB9067). CNTF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-CNTF Antibody (PB9067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of CNTF using anti-CNTF antibody (PB9067). CNTF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-CNTF Antibody (PB9067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-CNTF Antibody Picoband(r)
PB9067
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetCntf
Overview
- SupplierBoster Bio
- Product NameAnti-CNTF Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for CNTF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- Applications SupplierELI, IHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID25707
- Target nameCntf
- Target descriptionciliary neurotrophic factor
- Target synonymsciliary neurotrophic factor; ciliary neurotropic factor
- HostRabbit
- IsotypeIgG
- Protein IDP20294
- Protein NameCiliary neurotrophic factor
- Scientific DescriptionBoster Bio Anti-CNTF Antibody Picoband® catalog # PB9067. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Reactivity SupplierMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203