Anti-Collagenase type IV [3G11]

Absolute Antibody

Anti-Collagenase type IV [3G11]

9

This antibody is specific for type IV collagenase.

The immunoreactivity of the original format of this antibody (mouse IgG1, kappa) was measured via ELISA and was shown to decrease by approximately 10%-20% after cold iodination with 131-iodide. The (131)I-labeled version of this antibody was demonstrated to be stable in vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was defined as a two-compartment model, with a 7.2 h T1/2alpha and a 345.2 h T1/2beta, and clear scintigraphic images of human lung carcinoma PG were obtained using (125)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al., 2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of this antibody, and the resulting immunoconjugate maintained most of its immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells than free LDM by MTT assay. Furthermore, when administered intravenously, the Fab-LDM conjugate was proven to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings, increasing mice survival time (Fengqiang et al., 2004; PMID: 15382678). The scFv version of this antibody was constructed and used in various configurations for IF and in vivo imaging and therapeutic applications (Zhong et al., 2010; PMID: 20303650) (Kong et al., 2015; PMID: 25824464).

ImmunoFluorescence, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

3G11

Mmp2

matrix metallopeptidase 2

72 kDa gelatinase; 72 kDa type IV collagenase; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; Clg; Clg4a; Ge; GelA; gelatinase A; matrix metalloproteinase 2; MMP-; MMP-2

Mouse

IgG1

72 kDa type IV collagenase

Mouse

-20°C,2°C to 8°C

12352203