Figure 1. Western blot analysis of C5A using anti-C5A antibody (PB9187). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat C5A protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5A antigen affinity purified polyclonal antibody (Catalog # PB9187) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5A at approximately 37 kDa. The expected band size for C5A is at 37 kDa.
Figure 1. Western blot analysis of C5A using anti-C5A antibody (PB9187). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat C5A protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5A antigen affinity purified polyclonal antibody (Catalog # PB9187) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5A at approximately 37 kDa. The expected band size for C5A is at 37 kDa.
Anti-C5/C5a Antibody Picoband(r)
PB9187
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetC5
Overview
- SupplierBoster Bio
- Product NameAnti-complement C5a Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for C5a is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID362119
- Target nameC5
- Target descriptioncomplement C5
- Target synonymsanaphylatoxin; C5a; complement C5; complement component 5; Hc; RGD1561905
- HostRabbit
- IsotypeIgG
- Protein IDP08650
- Protein NameComplement C5
- Scientific DescriptionBoster Bio Anti-C5/C5a Antibody Picoband® catalog # PB9187. Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Reactivity SupplierRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203