IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026). Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026). Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Connexin 43/GJA1 Antibody Picoband(r)
PA1026
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetGJA1
Overview
- SupplierBoster Bio
- Product NameAnti-Connexin 43/GJA1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2697
- Target nameGJA1
- Target descriptiongap junction protein alpha 1
- Target synonymsAVSD3; CMDR; connexin-43; CX43; EKVP; EKVP3; gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa; GJAL; HLHS1; HSS; ODDD; PPKCA
- HostRabbit
- IsotypeIgG
- Protein IDP17302
- Protein NameGap junction alpha-1 protein
- Scientific DescriptionBoster Bio Anti-Connexin 43/GJA1 Antibody catalog # PA1026. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityChicken, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Chicken
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats. Li S et al., 2021 Sep, Environ Sci Pollut Res IntRead more