Figure 1. Western blot analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (PB9624). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Gaster Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 45/GJA7 antigen affinity purified polyclonal antibody (Catalog # PB9624) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 45/GJA7 at approximately 45 kDa. The expected band size for Connexin 45/GJA7 is at 45 kDa.
Figure 1. Western blot analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (PB9624). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Gaster Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 45/GJA7 antigen affinity purified polyclonal antibody (Catalog # PB9624) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 45/GJA7 at approximately 45 kDa. The expected band size for Connexin 45/GJA7 is at 45 kDa.
Anti-Connexin 45/GJA7/GJC1 Antibody Picoband(r)
PB9624-DYLIGHT550
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetGJC1
Overview
- SupplierBoster Bio
- Product NameAnti-Connexin 45/GJA7/GJC1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 550
- Gene ID10052
- Target nameGJC1
- Target descriptiongap junction protein gamma 1
- Target synonymsconnexin-45; CTC-296K1.4; CX45; gap junction alpha-7 protein; gap junction gamma-1 protein; gap junction protein, gamma 1, 45kDa; GJA7
- HostRabbit
- IsotypeIgG
- Protein IDP36383
- Protein NameGap junction gamma-1 protein
- Scientific DescriptionBoster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # PB9624. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203