Anti-Cortisol [5A4]

Absolute Antibody

Anti-Cortisol [5A4]

9

This antibody binds cortisol. It has also shown cross-reactivity to dexamethasone and prednisolone. This is due to a hydroxyl group bound to C11. Cortisol is a steroid hormone, in the glucocorticoid class of hormones. It is produced in many animals, mainl

The specificity and affinity of this mouse version of this antibody was determined using equilibrium dialysis in dialysis cells fitted with a cellulose membrane of 3500 MW cutoff (Calvez et al, 1995; pmid:7898495). Further a radioimmunoassay was performed on I125-cortisol using the scFv version of this antibody (Calvez et al, 1995; pmid:7898495). The specificity of this antibody towards cortisol-3-CMO-BSA, cortisol, prednisolone and dexamethasone was also tested using equilibrium dialysis. ELISA was used to show that this clone was able to bind steroids conjugated to BSA through nonspecific hydrophobic interactions (PMID: 9820517).

ELISA, RadioImmunoAssay

Research Use Only

Monoclonal

5A4

Mouse

IgG1

Other Species

-20°C,2°C to 8°C

12352203