Anti-Cotinine [CT#45]

Absolute Antibody

Anti-Cotinine [CT#45]

7

This antibody binds cotinine. Cotinine is an alkaloid found in tobacco and is also the predominant metabolite of nicotine. It is used as a biomarker for exposure to tobacco smoke.

This antibody was used in the development of an ELISA for determining urinary cotinine to monitor tobacco smoke exposure. This ELISA produced dose-response curves for cotinine ranging from 0.40-100 ng per assay and was able to discriminate nicotine, cotinine N-oxide, cotinine N-glucuronide (cross-reactivity was each <0.5%, taking cotinine as 100%), (3R, 5S)-3-hydroxycotinine O-glucuronide (0.6%) and (R, S)-norcotinine (1.5%). Another major nicotine metabolite, (3R, 5S)-3-hydroxycotinine, showed 8.4% cross-reactivity with this antibody (Kobayashi, 2011)

ELISA

ELISA

Research Use Only

Monoclonal

CT#45

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original format for improved compatibility with existing reagents assays and techniques.

Other Species

Species Independent

The original antibody was generated by immunizing BALB/c mice with a cotinine–albumin conjugate.

-20°C,2°C to 8°C

12352203