![Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD. Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.](https://www.bosterbio.com/media/catalog/product/p/a/pa1852-ataxin_3-primary-antibodies-wb-testing-2.jpg)
Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
Anti-Coxsackie Adenovirus Receptor Antibody
![Research Use Only](static/images/certificates/ruo.jpg)
PA1852
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Coxsackie Adenovirus Receptor Antibody
- Delivery Days Customer9
- Applications SupplierWB
- CertificationResearch Use Only
- ConcentrationAdd 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Estimated PurityImmunogen affinity purified.
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- UNSPSC12352203