Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (PB9966). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: rat testis tissue lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse skeletal muscle tissue lysates, Lane 14: mouse lung tissue lysates, Lane 15: mouse testis tissue lysates, Lane 16: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRM1 antigen affinity purified polyclonal antibody (Catalog # PB9966) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (PB9966). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: rat testis tissue lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse skeletal muscle tissue lysates, Lane 14: mouse lung tissue lysates, Lane 15: mouse testis tissue lysates, Lane 16: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRM1 antigen affinity purified polyclonal antibody (Catalog # PB9966) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Anti-CRM1/XPO1 Antibody Picoband(r)
PB9966
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetXPO1
Overview
- SupplierBoster Bio
- Product NameAnti-CRM1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID7514
- Target nameXPO1
- Target descriptionexportin 1
- Target synonymschromosome region maintenance 1 homolog; chromosome region maintenance 1 protein homolog; CRM1; CRM-1; emb; exp1; exportin 1 (CRM1 homolog, yeast); exportin-1; exportin-1 (required for chromosome region maintenance)
- HostRabbit
- IsotypeIgG
- Protein IDO14980
- Protein NameExportin-1
- Scientific DescriptionBoster Bio Anti-CRM1/XPO1 Antibody Picoband® catalog # PB9966. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203