Anti-CSAp [Mu-9]

Absolute Antibody

Anti-CSAp [Mu-9]

9

This antibody binds to a single site epitope of the CSAp antigen expressed on the GW-39 tumor cells.

This antibody binds a single site epitope of the CSAp and has an afiinity constant Ka = 9.61 x 108/mol. This antibody is not suitable for sandwich ELISA as it binds to a single site epitope of the CSAp, it is however suitable for direct and competition ELISA. This antibody does not react with reduced and alkylated CSAp. This antibody cannot fully block the reactivity of goat anti-CSAp. Immunohistochemical staining of neoplastic tissues showed the Mu-9-reactive determinant to be present in 60% of colon carcinomas. This antibody showed no reactivity outside the intestinal tract and no reactivity with blood cells (PMID: 2169346). The comparison of therapeutic efficacy and host toxicity of two different 131I-labelled antibodies effects of antibodies, Mu-9 and NP-4 IgG (anti-CEA antibody) revealed that even though the anti tumor effects of both these antibodies are equal, there is a significant difference in their therapeutic effectiveness (PMID: 2759735). Radioimmunotherapy of GW-39 human colonic tumor xenografts grown in the hamster cheek pouch with131I-labeled NP-4 anti-(carcinoembryonic antigen) (CEA) and131I-labeled Mu-9 anti-(color-specific antigen-p) (CSAp) murine monoclonal antibodies, administered in combination, was more effective than using either antibody alone for tumor masses less than 0.5 cm3 in size (PMID: 1652355). Phase I clinical trials of radiolabelled antibody and fragment revealed that both the 131I-Mu-9 IgG and the 131I-F(ab)2 were cleared from the blood successfully. Eight of nine patients given the whole immunoglobulin G developed highly elevated levels of human anti-mouse antibody, whereas lower values were observed in five of seven patients given the F(ab)2. Both the antibody and F(ab)2 were chosen for further evaluation for targeting gastrointestinal cancer for radioimmunotherapy (PMID: 8306272). The humanized version of this antibody was found to have immunoreactivity that is comparable to the original murine version.

ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

Mu-9

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203