Figure 1. Western blot analysis of IP10 using anti-IP10 antibody (PB9695). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP10 antigen affinity purified polyclonal antibody (Catalog # PB9695) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP10 at approximately 34 kDa. The expected band size for IP10 is at 11 kDa.
Figure 1. Western blot analysis of IP10 using anti-IP10 antibody (PB9695). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP10 antigen affinity purified polyclonal antibody (Catalog # PB9695) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP10 at approximately 34 kDa. The expected band size for IP10 is at 11 kDa.
Anti-IP10/CXCL10 Antibody Picoband(r)
PB9695
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCXCL10
Overview
- SupplierBoster Bio
- Product NameAnti-CXCL10/IP-10 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3627
- Target nameCXCL10
- Target descriptionC-X-C motif chemokine ligand 10
- Target synonyms10 kDa interferon gamma-induced protein; C7; crg-2; C-X-C motif chemokine 10; gamma IP10; gIP-10; IFI10; INP10; interferon-inducible cytokine IP-10; IP-10; mob-1; protein 10 from interferon (gamma)-induced cell line; SCYB10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10
- HostRabbit
- IsotypeIgG
- Protein IDP02778
- Protein NameC-X-C motif chemokine 10
- Scientific DescriptionBoster Bio Anti-IP10/CXCL10 Antibody Picoband® catalog # PB9695. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203