IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-CXCL12 Antibody
A00053-2
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-CXCL12 Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203