Figure 1. Western blot analysis of CXCL13 using anti-CXCL13 antibody (PB9697). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13 antigen affinity purified polyclonal antibody (Catalog # PB9697) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13 at approximately 13KD. The expected band size for CXCL13 is at 13KD.
Figure 1. Western blot analysis of CXCL13 using anti-CXCL13 antibody (PB9697). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13 antigen affinity purified polyclonal antibody (Catalog # PB9697) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13 at approximately 13KD. The expected band size for CXCL13 is at 13KD.
Anti-BCA1/CXCL13 Antibody Picoband(r)
PB9697
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCXCL13
Overview
- SupplierBoster Bio
- Product NameAnti-CXCL13/BLC Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID10563
- Target nameCXCL13
- Target descriptionC-X-C motif chemokine ligand 13
- Target synonymsANGIE; ANGIE2; b cell-attracting chemokine 1; b lymphocyte chemoattractant; BCA1; BCA-1; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); CXC chemokine BLC; C-X-C motif chemokine 13; SCYB13; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); small-inducible cytokine B13
- HostRabbit
- IsotypeIgG
- Protein IDO43927
- Protein NameC-X-C motif chemokine 13
- Scientific DescriptionBoster Bio Anti-BCA1/CXCL13 Antibody Picoband® catalog # PB9697. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203