Figure 1. Western blot analysis of CXCL13/BLC using anti-CXCL13/BLC antibody (PB9999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse CXCL13/BLC protein 1ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13/BLC antigen affinity purified polyclonal antibody (Catalog # PB9999) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13/BLC at approximately 13 kDa. The expected band size for CXCL13/BLC is at 12 kDa.
Figure 1. Western blot analysis of CXCL13/BLC using anti-CXCL13/BLC antibody (PB9999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse CXCL13/BLC protein 1ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13/BLC antigen affinity purified polyclonal antibody (Catalog # PB9999) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13/BLC at approximately 13 kDa. The expected band size for CXCL13/BLC is at 12 kDa.
Anti-BCA1/CXCL13 Antibody Picoband(r)
PB9999
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCxcl13
Overview
- SupplierBoster Bio
- Product NameAnti-CXCL13/BLC Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID55985
- Target nameCxcl13
- Target descriptionchemokine (C-X-C motif) ligand 13
- Target synonyms4631412M08Rik; ANG; Angie; ANGIE2; B lymphocyte chemoattractant; BCA-; BCA-1; BL; BLC; BLR1L; CXC chemokine BLC; C-X-C motif chemokine 13; Scyb1; Scyb13; small inducible cytokine subfamily B (Cys-X-Cys), member 13; small-inducible cytokine B13
- HostRabbit
- IsotypeIgG
- Protein IDO55038
- Protein NameC-X-C motif chemokine 13
- Scientific DescriptionBoster Bio Anti-BCA1/CXCL13 Antibody Picoband® catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203