Figure 1. Western blot analysis of CXCL5 using anti-CXCL5 antibody (PB9859). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat CXCL5 protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL5 antigen affinity purified polyclonal antibody (Catalog # PB9859) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL5 at approximately 14 kDa. The expected band size for CXCL5 is at 14 kDa.
Figure 1. Western blot analysis of CXCL5 using anti-CXCL5 antibody (PB9859). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat CXCL5 protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL5 antigen affinity purified polyclonal antibody (Catalog # PB9859) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL5 at approximately 14 kDa. The expected band size for CXCL5 is at 14 kDa.
Anti-CXCL5 Antibody Picoband(r)
PB9859
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetCxcl6
Overview
- SupplierBoster Bio
- Product NameAnti-CXCL5/ENA-78 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID60665
- Target nameCxcl6
- Target descriptionC-X-C motif chemokine ligand 6
- Target synonymschemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); CXC chemokine LIX; C-X-C motif chemokine 5; Cxcl5; cytokine LIX; small-inducible cytokine B5
- HostRabbit
- IsotypeIgG
- Protein IDP97885
- Protein NameC-X-C motif chemokine 5
- Scientific DescriptionBoster Bio Anti-CXCL5 Antibody Picoband® catalog # PB9859. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203