Western blot analysis of CYBB expression in MCF-7 cell lysate (M00328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYBB monoclonal antibody (Catalog # M00328) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYBB
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
M00328
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYBB
Overview
- SupplierBoster Bio
- Product NameAnti-CYBB/Nox2 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGOH-3
- FormulationLiquid
- Gene ID1536
- Target nameCYBB
- Target descriptioncytochrome b-245 beta chain
- Target synonymsAMCBX2; CGD; CGD91-phox; CGDX; cytochrome b(558) subunit beta; cytochrome b-245 beta polypeptide; cytochrome b-245 heavy chain; cytochrome b558 subunit beta; GP91-1; GP91PHOX; GP91-PHOX; heme-binding membrane glycoprotein gp91phox; IMD34; NADPH oxidase 2; neutrophil cytochrome b 91 kDa polypeptide; NOX2; p22 phagocyte B-cytochrome; p91-PHOX; superoxide-generating NADPH oxidase heavy chain subunit
- HostRabbit
- IsotypeIgG
- Protein IDP04839
- Protein NameCytochrome b-245 heavy chain
- Scientific DescriptionBoster Bio Anti-CYBB/Nox2 Rabbit Monoclonal Antibody catalog # M00328. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203