Figure 1. Western blot analysis of CCN1 using anti-CCN1 antibody (PB9549). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: A431 Whole Cell Lysate at 40ug, Lane 4: SGC Whole Cell Lysate at 40ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1 antigen affinity purified polyclonal antibody (Catalog # PB9549) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCN1 at approximately 42 kDa. The expected band size for CCN1 is at 42 kDa.
Figure 1. Western blot analysis of CCN1 using anti-CCN1 antibody (PB9549). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: A431 Whole Cell Lysate at 40ug, Lane 4: SGC Whole Cell Lysate at 40ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1 antigen affinity purified polyclonal antibody (Catalog # PB9549) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCN1 at approximately 42 kDa. The expected band size for CCN1 is at 42 kDa.
Anti-CCN1/CYR61 Antibody Picoband(r)
PB9549
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetCCN1
Overview
- SupplierBoster Bio
- Product NameAnti-CYR61/CCN1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3491
- Target nameCCN1
- Target descriptioncellular communication network factor 1
- Target synonymsCCN family member 1; CYR61; cysteine rich angiogenic inducer 61; cysteine-rich heparin-binding protein 61; cysteine-rich, anigogenic inducer, 61; GIG1; IBP-10; IGF-binding protein 10; IGFBP10; IGFBP-10; insulin-like growth factor-binding protein 10; protein CYR61
- HostRabbit
- IsotypeIgG
- Protein IDO00622
- Protein NameProtein CYR61
- Scientific DescriptionBoster Bio Anti-CCN1/CYR61 Antibody Picoband® catalog # PB9549. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203