Figure 2. IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335). Cytokeratin 19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335). Cytokeratin 19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Cytokeratin 19/KRT19 Antibody Picoband(r)
PA1335-10UG
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey
TargetKRT19
Overview
- SupplierBoster Bio
- Product NameAnti-Cytokeratin 19/KRT19 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID3880
- Target nameKRT19
- Target descriptionkeratin 19
- Target synonyms40-kDa keratin intermediate filament; CK19; CK-19; cytokeratin 19; K19; K1CS; keratin 19, type I; keratin, type I cytoskeletal 19; keratin, type I, 40-kd
- HostRabbit
- IsotypeIgG
- Protein IDP08727
- Protein NameKeratin, type I cytoskeletal 19
- Scientific DescriptionBoster Bio Anti-Cytokeratin 19/KRT19 Antibody catalog # PA1335. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203