Bio-Connect
Immunohistochemical staining of formalin fixed and paraffin embedded human Prostate tissue section using anti-CK-5/CK-6 rabbit monoclonal antibody (Clone RM341) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human Prostate tissue section using anti-CK-5/CK-6 rabbit monoclonal antibody (Clone RM341) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human Prostate tissue section using anti-CK-5/CK-6 rabbit monoclonal antibody (Clone RM341) at a 1:1000 dilution.

anti-Cytokeratin-5 / -6 (human), Rabbit Monoclonal (RM341)

REV-31-1227-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetKRT5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-Cytokeratin-5 / -6 (human), Rabbit Monoclonal (RM341)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM341
  • Gene ID3852
  • Target name
    KRT5
  • Target description
    keratin 5
  • Target synonyms
    58 kda cytokeratin; CK5; CK-5; cytokeratin-5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin, type II cytoskeletal 5; KRT5A; type-II keratin Kb5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13647
  • Protein Name
    Keratin, type II cytoskeletal 5
  • Scientific Description
    Cytokeratins are keratin proteins found in the intracytoplasmic cytoskeleton of epithelial tissue (at least 20 different polypeptides). They are an important component of intermediate filaments, which help cells resist mechanical stress. Expression of these cytokeratins within epithelial cells is largely specific to particular organs or tissues. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus a specific cytokeratin expression profile allows the identification of epithelial cells. Furthermore, this applies also to the malignant counterparts of the epithelia, (carcinomas). Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin-5/-6 are type II cytokeratins specifically expressed in the basal layer of the epidermis. Targeting both cytokeratin 5 and cytokeratin 6 is used in immunohistochemistry. This often called CK5/6 staining is used for identifying basal cells or myoepithelial cells in the breast and prostate, for breast pathology in distinguishing usual ductal hyperplasia (UDH) and papillary lesions from ductal carcinoma in situ, to distinguish between the diagnosis of papilloma or papillary carcinoma, and in the lung, distinguishing epithelioid mesothelioma (CK5/6 positive in 83%) from lung adenocarcinoma (CK5/6 negative in 85%). - Recombinant Antibody. This antibody reacts to both human CK-5 (Cytokeratin 5) and CK-6 (Cytokeratin 6). Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Cytokeratins are keratin proteins found in the intracytoplasmic cytoskeleton of epithelial tissue (at least 20 different polypeptides). They are an important component of intermediate filaments, which help cells resist mechanical stress. Expression of these cytokeratins within epithelial cells is largely specific to particular organs or tissues. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus a specific cytokeratin expression profile allows the identification of epithelial cells. Furthermore, this applies also to the malignant counterparts of the epithelia, (carcinomas). Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin-5/-6 are type II cytokeratins specifically expressed in the basal layer of the epidermis. Targeting both cytokeratin 5 and cytokeratin 6 is used in immunohistochemistry. This often called CK5/6 staining is used for identifying basal cells or myoepithelial cells in the breast and prostate, for breast pathology in distinguishing usual ductal hyperplasia (UDH) and papillary lesions from ductal carcinoma in situ, to distinguish between the diagnosis of papilloma or papillary carcinoma, and in the lung, distinguishing epithelioid mesothelioma (CK5/6 positive in 83%) from lung adenocarcinoma (CK5/6 negative in 85%).
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetKRT5
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Western Blot analysis of HepG2 cells using Cytokeratin 5 Polyclonal Antibody
Product group Antibodies
Cusabio
KRT5 AntibodyCSB-PA002049
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetKRT5
  • SizePrice
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti Cytokeratin 5/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT5
  • SizePrice
Immunohistochemistry analysis in human prostate and kidney tissues using AMAb91549 antibody. Corresponding KRT5 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetKRT5
  • SizePrice
Product group Antibodies
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT5
  • SizePrice
Figure 1. Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 5 antigen affinity purified polyclonal antibody (Catalog # A00398) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetKRT5
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetKRT5
  • SizePrice
Immunohistochemical staining of formalin fixed and paraffin embedded human Lung Squamous Cell Carcinoma tissue section using anti-CK-5 rabbit monoclonal antibody (Clone RM226) at a 1:200 dilution.
Product group Antibodies
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetKRT5
  • SizePrice
Anti-Cytokeratin 5 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • SizePrice