More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Daxx Antibody Picoband(r)
Figure 1. Western blot analysis of DAXX using anti-DAXX antibody (PA1809). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAXX antigen affinity purified polyclonal antibody (Catalog # PA1809) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAXX at approximately 115 kDa. The expected band size for DAXX is at 81 kDa.
Figure 1. Western blot analysis of DAXX using anti-DAXX antibody (PA1809). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAXX antigen affinity purified polyclonal antibody (Catalog # PA1809) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAXX at approximately 115 kDa. The expected band size for DAXX is at 81 kDa.
Figure 1. Western blot analysis of DAXX using anti-DAXX antibody (PA1809). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAXX antigen affinity purified polyclonal antibody (Catalog # PA1809) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAXX at approximately 115 kDa. The expected band size for DAXX is at 81 kDa.

Anti-Daxx Antibody Picoband(r)

Research Use Only
PA1809-APC
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetDAXX
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Daxx Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID1616
  • Target name
    DAXX
  • Target description
    death domain associated protein
  • Target synonyms
    BING2; CENP-C binding protein; DAP6; death domain-associated protein 6; death-associated protein 6; EAP1; ETS1-associated protein 1; fas death domain-associated protein; Fas-binding protein; SMIM40
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UER7
  • Protein Name
    Death domain-associated protein 6
  • Scientific Description
    Boster Bio Anti-Daxx Antibody catalog # PA1809. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203