Figure 1. IHC analysis of DCC using anti-DCC antibody (A00560). DCC was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of DCC using anti-DCC antibody (A00560). DCC was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-DCC Antibody Picoband(r)
A00560-BIOTIN
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDCC
Overview
- SupplierBoster Bio
- Product NameAnti-DCC Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID1630
- Target nameDCC
- Target descriptionDCC netrin 1 receptor
- Target synonymscolorectal cancer suppressor; CRC18; CRCR1; deleted in colorectal carcinoma; HGPPS2; IGDCC1; immunoglobulin superfamily DCC subclass member 1; MRMV1; netrin receptor DCC; NTN1R1; putative colorectal tumor suppressor; tumor suppressor protein DCC
- HostRabbit
- IsotypeIgG
- Protein IDP43146
- Protein NameNetrin receptor DCC
- Scientific DescriptionBoster Bio Anti-DCC Antibody Picoband® catalog # A00560. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203