Figure 1. Western blot analysis of DDB2 using anti-DDB2 antibody (PB9757). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: SW620 Whole Cell Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: JURKAT Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # PB9757) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48 kDa. The expected band size for DDB2 is at 48 kDa.
Figure 1. Western blot analysis of DDB2 using anti-DDB2 antibody (PB9757). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: SW620 Whole Cell Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: JURKAT Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # PB9757) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48 kDa. The expected band size for DDB2 is at 48 kDa.
Anti-DDB2 Antibody Picoband(r)
PB9757-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDDB2
Overview
- SupplierBoster Bio
- Product NameAnti-DDB2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID1643
- Target nameDDB2
- Target descriptiondamage specific DNA binding protein 2
- Target synonymsdamage-specific DNA binding protein 2, 48kDa; DDB p48 subunit; DDBB; DNA damage-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XPE
- HostRabbit
- IsotypeIgG
- Protein IDQ92466
- Protein NameDNA damage-binding protein 2
- Scientific DescriptionBoster Bio Anti-DDB2 Antibody Picoband® catalog # PB9757. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203