Western blot analysis of DDX5 expression in HeLa cell lysate using anti-DDX5 antibody (M00670). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX5 antigen affinity purified polyclonal antibody (Catalog # M00670) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for DDX5.
Anti-DDX5 Monoclonal Antibody
M00670
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDX5
Overview
- SupplierBoster Bio
- Product NameAnti-DDX5 Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDADBC-4
- FormulationLiquid
- Gene ID1655
- Target nameDDX5
- Target descriptionDEAD-box helicase 5
- Target synonymsATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); G17P1; HLR1; HUMP68; p68; probable ATP-dependent RNA helicase DDX5; RNA helicase p68
- HostRabbit
- IsotypeIgG
- Protein IDP17844
- Protein NameProbable ATP-dependent RNA helicase DDX5
- Scientific DescriptionBoster Bio Anti-DDX5 Monoclonal Antibody catalog # M00670. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203