Anti-deltaEGFR [DH8.3]

Absolute Antibody

Anti-deltaEGFR [DH8.3]

9

This antibody is specific for deltaEGFR (EGFR type III), a truncated form of the epidermal growth factor receptor that is commonly associated with cancer cells, especially glioblastoma cells. deltaEGFR lacks exons 2-7 of the external domain. This antibody

This antibody has been used in fluorescence-activated cell sorting, Western blot and immunohistochemical analysis, using acetone-fixed frozen sections, of a tumour panel (Jungbluth et al, 2003). It does not stain normal tissues, which express wild-type EGFR (Jungbluth et al, 2003), and preferentially stains tumours expressing mutant receptors (Hills et al, 1995). This antibody has also been used in immunoprecipitation experiments (Hills et al, 1995) and ELISA analysis (Johns et al, 2002). This antibody has been shown to induce rapid internalisation of truncated EGFR by target cells (Johns et al, 2002), which could have therapeutic applications, given the tumour-restricted expression of deltaEGFR and its role in tumorigenesis.

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

DH8.3

EGFR

epidermal growth factor receptor

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Rabbit

IgG

Epidermal growth factor receptor

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203