Anti-Desmoglein 3 [AK23]

Absolute Antibody

Anti-Desmoglein 3 [AK23]

9

This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that

This antibody can be used to study the blister formation and complex interaction that occur in autoimmune blistering disease like Pemphigus vulgaris (PV). Pemphigus vulgaris (PV) is an epidermal blistering disorder caused by antibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). AK23 was reported to induce pemphigus vulgaris (PV) blisters in neonatal mice. The binding affinity of this antibody for human and mouse desmoglein 3 was determined using ELISA. This antibody was also used in the live keratinocyte staining of mouse PAM212 cell line and indirect immunofluorescent staining of mouse oral mucuous membrance, mouse liver and lower parts of the human epidermis. Immunoprecipitaton was used for the determination of the epitope of the antibody (PMID: 12574390). This antibody was also used in the study of alteration in desmosome morphology (PMID: 23226536).

ImmunoFluorescence, ImmunoPrecipitation, ELISA

Research Use Only

Monoclonal

AK23

DSG3

desmoglein 3

130 kDa pemphigus vulgaris antigen; ABOLM; cadherin family member 6; CDHF6; desmoglein-3; PVA

Mouse

IgG1

Desmoglein-3

Human, Mouse

-20°C,2°C to 8°C

12352203