Anti-Desmoglein-3 [F779]

Absolute Antibody

Anti-Desmoglein-3 [F779]

9

This antibody binds human desmoglein-3 between amino acids 1-161 and does not cross react with desmoglein-1. It is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating

This antibody can be used for the determination of desmoglein-3 using ELISA. The binding affinity of IgG1 version of this antibody for human desmoglein-3 was determined using surface plasmon resonance and was found to be Kd = 1.77 nM (PMID: 24942562). This antibody was also used in the direct and indirect immunofluorescence staining of frozen human skin sections (PMID: 24942562; 27304671) The IgG1 and IgG4 variants of this antibody induce loss of cell surface Dsg3 in primary human epidermal keratinocytes and suprabasal blisters in human skin (PMID: 27304671). This antibody is pathogenic in nature and caused suprabasal blisters in human skin at comparable doses of 25 microg and 50 microg (PMID: 27304671).

ImmunoFluorescence, ELISA

Research Use Only

Monoclonal

F779

DSG3

desmoglein 3

130 kDa pemphigus vulgaris antigen; ABOLM; cadherin family member 6; CDHF6; desmoglein-3; PVA

Mouse

IgG1

Desmoglein-3

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203