Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads

PTM BIO

Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads

5

With the immobilization of highly specific Anti-Diglycyl-Lysine Antibody, the Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion or remnants of SUMO after alfa-lytic protease WaLP digestion. This product has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation.

IAP

Antibody

Research Use Only

Mix

Agarose

IgG

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminus and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule.Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IkappaB-alfa. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress.

Stable for 12 months from date of receipt.

All Species

All

Store at -20°C. Avoid freeze/thaw cycles.

12352203