Anti-Digoxin [26 / 10]

Absolute Antibody

Anti-Digoxin [26 / 10]

7

The antibody binds digoxin with an affinity (Kd) of 9 nM.

The antibody is specific for digoxin, a cardiac glycoside derived from the foxglove plant. Digoxin is administered for the therapeutic treatment of heart conditions such as atrial fibrillation, atrial flutter and heart failure, an acts by inhibiting the Na+/K+ ATPase. However, the drug has a very narrow therapeutic index and can cause toxic side effects. The Fab fragment prepared from 26-10 that neutralises the activity of digoxin and can be used as an antidote in case of overdose of digitalis. Anti-digoxin monoclonal antibodies can also be used to measure serum levels of the drug as a guide to therapy, and are useful in the investigation of the kinetics of interaction between antibody and antigen.

ELISA, RadioImmunoAssay

ELISA; RIA

Research Use Only

Monoclonal

26 / 10

Human

IgG1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Plant

Digitalis lanata

Spleen cells from A/J mice immunized with digoxin coupled to human serum albumin (Dig-HSA).

-20°C,2°C to 8°C

12352203