Anti-Disialoganglioside 2 & 3 [ME36.1]

Absolute Antibody

Anti-Disialoganglioside 2 & 3 [ME36.1]

7

This antibody binds both GD2 and GD3. ME36.1 has higher affinity for GD2 than GD3.

The disialoganglioside GD2 is expressed on the surface of cells of most human melanomas but not on the surface of melanocytes. The specificty of this antibody towards ganglioside antigens GD2 and GD3 was demonstrated using solid-phase radioimmunoassay and enzyme-linked immunosorbent assay as well as by immunostaining on thin-layer chromatography plates using structurally characterized gangliosides. Binding of antibody to live cells was done by flow cytometry and by immunoperoxidase staining on frozen tissue sections. The binding patterns correlated with antitumor activity in antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity assays with human effector cells and complement in an release assay using cell lines derived from the same individual. (PMID: 3815333). This antibody was also used in monitering the levels of GD2 and GD3 in the sera of patients suffering from melanoma and healthy adults (PMID: 2778837)

Flow Cytometry, ELISA, RadioImmunoAssay

RIA; ELISA; Immunoperoxidase staining; FC

Research Use Only

Monoclonal

ME36.1

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing BLAB/c mice with cells of metastatic melanoma SK MEL 23 cell culture and boosted again four weeks later.

-20°C,2°C to 8°C

12352203