Anti-DLL3 [SC16.13]

Absolute Antibody

Anti-DLL3 [SC16.13]

7

This antibody binds human DLL3 in the EGF2 domain. It does not cross-react with monkey/mouse/rat DLL3. Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL3 consists

The binding characterization of this antibody to human DLL3 was done using ELISA. Binding affinity of mouse IgG antibody for human DLL3 is 0.3 nM. This antibody can bind human DLL3 expressed on surface of 293 cells via flow cytometry. This antibody can mediate killing of tumor cells expressing DLL3. This antibody was covalently conjugated to PBD cytotoxic agent and the resulting ADCs resulted in tumor growth suppression lasting as long as 20 days. Humanized version is also available and drug conjugations made using the humanized versions also resulted in tumor suppression (US9089615).

Functional Assay, Flow Cytometry, ELISA

ELISA; FC; functional assay

Research Use Only

Monoclonal

SC16.13

DLL3

delta like canonical Notch ligand 3

delta3; delta-like 3; delta-like protein 3; drosophila Delta homolog 3; SCDO1

Rabbit

IgG

Delta-like protein 3

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing three strains of mice (Balb/c, CD-1 and FVB) with recombinant human DLL3-Fc or with human DLL3-His fusion constructs.

-20°C,2°C to 8°C

12352203