Anti-domoic acid [2H12]

Absolute Antibody

Anti-domoic acid [2H12]

9

This antibody is specific for domoic acid. Domoic acid is a kainic acid-type neurotoxin that causes amnesic shellfish poisoning. It is produced by algae and accumulates in shellfish, sardines, and anchovies. When sea lions, otters, cetaceans, humans, and

The specificity of the original format and the scFv format of the antibody was confirmed by ELISA analysis (IC50 = 1 microg/ml and 245 ng/ml respectively, and Kd = 0.8 microM for the scFv fragment) (Hu et al., 2005; PMID: 16019098). The scFv was employed for the development of a novel immuno-sensor for in situ domoic acid screening, where the fragment was immobilized on mesoporous silica. Domoic acid binding analysis found that the adsorbed scFv bound domoic acid in a concentration-dependent manner (Hu et al., 2007; PMID: 17005419). The scFv antibody fragment was engineered with cysteine-containing linkers of two different lengths. The domoic acid detection ability of the covalently immobilized antibody fragments was compared with that of scFvs adsorbed non-covalently onto polystyrene plates. The IC50 for DA of the unmodified scFv in solution was previously reported as 245 ng/mL. In contrast, in this study, the scFv, 2H12 scFv-cys I, and 2H12 scFv-cys II proteins adsorbed onto standard Maxisorp plates were calculated to have IC50s for DA of 540 ng/mL, 1126 ng/mL and 1482 ng/mL, respectively, as measured by competitive ELISA (Hortigueela et al., 2013; PMID: 23493076).

ELISA

Research Use Only

Monoclonal

2H12

Rabbit

IgG

Other Species

-20°C,2°C to 8°C

12352203